Microbiology for Developing the Culture and Storing

Question: Discuss about a man with a surgical wound after a prosthetic hip placement? Answer: Introduction Appropriate lab practices can save lives and improve the quality of life (Hakim et al., 2014). In this case, if appropriate collection, safety and culture conditions were maintained then the patient would not have suffered repeated bouts of pain. This report discusses the optimal practices in the lab which can help maintain the safekeeping standards. Specimen collection protocol The specimen collection protocol should be followed, the type of specimen should be recognized, after collection the specimen should be transported in the appropriate container. In the hospital, the collection environment should be maintained (Crouch, 2014). In this case, the joint aspirate examination was performed with caution which revealed cloudy synovial fluid. Laboratory safety Personal and professional levels of lab safety should be maintained in the lab to ensure that every sample that comes in for testing is tested properly, no contaminant is mixed in the process, and the person performing the test is not affected by the infection. In the case discussed, high blood pressure, blister on the wound and cloudy synovial fluid reveals that either a persistent infection is there or injury has not healed properly. Culture Condition The quality of the culture should be maintained to ensure accurate results. Environmental factors have to be considered while developing the culture and storing the culture. Causative Agents Infections can be caused due to a number of microorganisms. In this case, bacterial infection was not found in the gram staining report, but the presence of blisters on the wound indicates that there was some unidentified infectious agent at work. The continued pain and tenderness of the surgical wound may indicate infection or unhealed wound. Pathogenesis The infection can spread from the operating theater or the home environment of the patient in case of infection after surgery. The infection can get transferred from the apparatus, clothes or bodies of individuals through tears, cuts, and wounds. Treatment The infections can be treated by surgical and non-surgical techniques. The mode of treatment depends on the severity of the infection, the effect of the infection and the expected result. Prevention Preventative measures are taken to ensure that surgical wounds are kept away from infections. The wound is kept clean and protected at all times. Conclusion Disease control and prevention is not an easy job. More importantly, infections after surgery should be avoided or treated properly. References Crouch, R. D. (2014, August). Reinforcing safety in the lab through a sequence of activities in the first four semesters of the chemistry curriculum. InABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY(Vol. 248). 1155 16TH ST, NW, WASHINGTON, DC 20036 USA: AMER CHEMICAL SOC. Hakim, J., Matenga, J., Borok, M., Mandinger, N., Ndemera, B., Taziwa, A. (2014). SIDE LAB MANUAL.